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Lab Test Dictionary

AFB Broad-Range PCR

Methodology

DNA Extraction; Nucleic Acid Purification; PCR; Sequencing


Synonyms

ABF; Acid Fast Bacili; Mycobacteria; Mycobacterial; Mycobacterium; MTB; TB

Specimen Type

See Collection Instructions

Performing Lab

U of Washington (UW) MC Molecular Microbiology


Epic ID

Other Reference Test

Lab Test Days

Monday-Friday

Standard TAT

2-4 Days


Collection Information

Specimen Required

1-2 mL Tissue or Body Fluid

Container Type

Sterile Container

Collection Instructions

Fresh frozen tissue collected in a sterile container or non-bloody body fluids collected in a sterile tube without preservative or in an EDTA (lavender-top) tube is acceptable. Formalin fixed paraffin embedded tissue (PET) is also acceptable. However, formalin fixation dramatically reduces the sensitivity of the assays due to reduced template yield and quality.


Processing Information - Lab Use Only

Transport Temperature

Frozen-Only

Rejection Criteria

Tissue in formalin; Samples containing SPS, citrate or heparin; Blood/Serum specimens; Samples collected on swabs

Test Volume Required

2 mL Tissue or Body Fluid

Minimum Test Volume

1 mL Tissue or Body Fluid

Processing Instructions

Complete a U of Washington Molecular Microbiology requisition and submit with specimen frozen. If submitting formalin fixed paraffin embedded tissue send room temp.


CPT Codes

87551, 87556


Revised

01-06-19

Initials

SL