Methodology
DNA Extraction; Nucleic Acid Purification; PCR; Sequencing
Synonyms
ABF; Acid Fast Bacili; Mycobacteria; Mycobacterial; Mycobacterium; MTB; TB
Specimen Type
See Collection Instructions
Performing Lab
U of Washington (UW) MC Molecular Microbiology
Epic ID
Other Reference Test
Lab Test Days
Monday-Friday
Standard TAT
2-4 Days
Collection Information
Specimen Required
1-2 mL Tissue or Body Fluid
Container Type
Sterile Container
Collection Instructions
Fresh frozen tissue collected in a sterile container or non-bloody body fluids collected in a sterile tube without preservative or in an EDTA (lavender-top) tube is acceptable. Formalin fixed paraffin embedded tissue (PET) is also acceptable. However, formalin fixation dramatically reduces the sensitivity of the assays due to reduced template yield and quality.
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Rejection Criteria
Tissue in formalin; Samples containing SPS, citrate or heparin; Blood/Serum specimens; Samples collected on swabs
Test Volume Required
2 mL Tissue or Body Fluid
Minimum Test Volume
1 mL Tissue or Body Fluid
Processing Instructions
Complete a U of Washington Molecular Microbiology requisition and submit with specimen frozen. If submitting formalin fixed paraffin embedded tissue send room temp.
CPT Codes
87551, 87556
Revised
01-06-19
Initials
SL