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Lab Test Dictionary

Bordetella pertussis and parapertussis, RT-PCR


Real-Time Polymerase Chain Reaction (RT-PCR)


B. Pertussis DNA; B.parapertussis DNA

Specimen Type

N/P Swab

Performing Lab

RCHSD-Discontinued as of 8/1/22

Epic ID


Lab Test Days


Standard TAT

1-2 Days

Collection Information

Specimen Required

Nasopharyngeal swab (Puritan-Sterile Polyester Tipped Applicator) in a sterile container with 0.5 mL sterile saline (lab supplied blue-top pertussis vial)

Container Type

Sterile Container

Collection Instructions

Collection vials and swabs are located in the lab. Insert N/P swab through one nostril into the posterior nasopharynx and rotate the swab, keeping it near the septum and floor of the nose. Repeat using the same swab for the other nostril. Place the entire swab into the collection tube, immersing the tip into the fluid and cap the tube.

Processing Information - Lab Use Only

Transport Temperature


Specimen Stability

Room Temp: Unacceptable; Refrigerated: 1 Week; Frozen: 1 Month

Rejection Criteria

Specimens with signs of leakage; Calcium alginate and wood swabs; Specimen submitted in viral transport media

Test Volume Required

0.5 mL

Minimum Test Volume

0.5 mL

Processing Instructions

Place specimen in -70 freezer in the PCR bin upon receipt if testing is not immediately performed.


For supplies needed for outside locations, contact Supply Chain Management (Carts) x225813 to place an order.

CPT Codes

87798 (x2)