Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Synonyms
B. Pertussis DNA; B.parapertussis DNA
Specimen Type
N/P Swab
Performing Lab
RCHSD-Discontinued as of 8/1/22
Epic ID
LAB952
Lab Test Days
Monday-Saturday
Standard TAT
1-2 Days
Collection Information
Specimen Required
Nasopharyngeal swab (Puritan-Sterile Polyester Tipped Applicator) in a sterile container with 0.5 mL sterile saline (lab supplied blue-top pertussis vial)
Container Type
Sterile Container
Collection Instructions
Collection vials and swabs are located in the lab. Insert N/P swab through one nostril into the posterior nasopharynx and rotate the swab, keeping it near the septum and floor of the nose. Repeat using the same swab for the other nostril. Place the entire swab into the collection tube, immersing the tip into the fluid and cap the tube.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: Unacceptable; Refrigerated: 1 Week; Frozen: 1 Month
Rejection Criteria
Specimens with signs of leakage; Calcium alginate and wood swabs; Specimen submitted in viral transport media
Test Volume Required
0.5 mL
Minimum Test Volume
0.5 mL
Processing Instructions
Place specimen in -70 freezer in the PCR bin upon receipt if testing is not immediately performed.
Comments
For supplies needed for outside locations, contact Supply Chain Management (Carts) x225813 to place an order.
CPT Codes
87798 (x2)
Revised
07-30-22
Initials
SA
© 2023 Rady Children's Hospital–San Diego