Methodology
Metagenomic Next-Generation Sequencing (NGS)
Synonyms
Pathogen Detection, mNGS, CSF (P710); mNGS, metagenomic next generation sequencing; CSF mNGS
Specimen Type
CSF
Performing Lab
Mayo Medical Lab
Epic ID
Other Reference Test
Lab Test Days
Monday-Friday
Standard TAT
7-14 Days
Collection Information
Specimen Required
0.5-2 mL CSF
Container Type
Sterile Container
Collection Instructions
1. Masks should be worn by those collecting and processing specimens for this assay. 2. Handle all vials under sterile technique when open to the air. 3. A separate collection vial of CSF is preferred. 4. Submit specimen from collection vial 2, 3, or 4, as specimens from vial 1 are not acceptable. 5. Indicate on the label which vial is being submitted. 6. Do not centrifuge or heat inactivate.
Processing Information - Lab Use Only
Transport Temperature
Refrigerated-OK/Frozen-OK
Specimen Stability
Room Temp: Unacceptable; Refrigerated: 7 Days; Frozen: 21 Days (preferred)
Rejection Criteria
Gross hemolysis; Shunt fluid; Heat-inactivated specimen
Test Volume Required
2 mL CSF
Minimum Test Volume
0.5 mL CSF
Processing Instructions
1. Masks should be worn by those collecting and processing specimens for this assay. 2. Handle all vials under sterile technique when open to the air. 3. A separate collection vial of CSF is preferred. 4. Submit specimen from collection vial 2, 3, or 4, as specimens from vial 1 are not acceptable. 5. Indicate on the label which vial is being submitted. 6. Do not centrifuge or heat inactivate. Order in Mayolink and send with Mayo courier Monday-Friday.
Comments
Detecting and identifying pathogenic organisms including bacteria, fungi, DNA viruses, RNA viruses, and parasites in cerebrospinal fluid.
CPT Codes
0480U
Revised
12/12/24
Initials
SA