Methodology
Enzyme- linked immunosorbent assay (ELISA)
Synonyms
IFN-ALPHA; IFN-A; INTERFERON-A; IFNALPHA
Specimen Type
Whole Blood or Plasma or Serum
Performing Lab
Cincinnati Children's Diagnostic Immunology
Epic ID
Other Reference Test
Lab Test Days
Every 2 Weeks
Standard TAT
2 Weeks
Collection Information
Specimen Required
1-3 mL Whole Blood
Container Type
EDTA (Lavender-Top) Tube
Collection Instructions
Serum collected in a red-top tube or gold serum separator tube (SST) is also acceptable. Keep sample as whole blood for delivery to reference lab within 24 hours of collection. If delivery cannot occur within 24 hours, separate plasma/serum from cells within 24 hours of collection and freeze immediately.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-OK/Frozen-OK
Specimen Stability
Room Temp: 24 Hours; Refrigerated: Unacceptable; Frozen: 6 Months (Plasma or Serum only) (Unacceptable for Whole Blood)
Rejection Criteria
Frozen whole blood; wrong container/tube type; serum/plasma sent room temp or refrigerated
Test Volume Required
3 mL Whole Blood or (two) 0.5 mL Serum or Plasma
Minimum Test Volume
1 mL Whole Blood or (one) 0.5 mL Serum or Plasma
Processing Instructions
Keep sample as whole blood for delivery to reference lab within 24 hours of collection. If delivery cannot occur within 24 hours, separate plasma/serum from cells within 24 hours of collection and freeze immediately. Complete a Cincinnati Children's Diagnostic Immunology requisition form and ship with sample room temp (whole blood only) or frozen (plasma or serum only).
CPT Codes
83520
Revised
7/19/23
Initials
SA