Methodology
Polymerase Chain Reaction
Synonyms
Biofire PNA Panel
Specimen Type
Bronchoalveolar Lavage (BAL)
Performing Lab
UCSD Microbiology
Epic ID
LAB07959
Lab Test Days
Daily
Standard TAT
1 Day
Collection Information
Specimen Required
1-2 mL Broncoalveolar Lavage (BAL)
Container Type
Sterile Container
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-Only
Specimen Stability
Room Temp=Unacceptable; Refrigerated=24 Hous; Frozen=Unacceptable
Rejection Criteria
Sputum; Endotracheal aspirates; Centrifuged or pre-processed samples; Specimens with preservatives, additives or mucolytic agents
Test Volume Required
2 mL Broncoalveolar Lavage (BAL)
Minimum Test Volume
1 mL Broncoalveolar Lavage (BAL)
Processing Instructions
Complete UCSD Microbiology/Virology requisition and submit with specimen refrigerated within 24 hours of collection. Send with TForce route courier Monday-Friday or call for a pick-up same day specimen is packaged including on weekends.
Included Tests
Detection of 18 Bacteria (Acinetobactercalcoaceticus-baumanii complex, Enterobacter cloacae complex, Escherichia coli, Haemophilus influenzae, Klebsiella aerogenes, Klebsiella oxytoca, Klebsiella pneumoniae group, Moraxella catarrhalis, Proteus spp., Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumonia), 7 resistance markers (CTX-M, IMP,
Comments
Because of the high sensitivity of this assay, it will not be performed on multiple BAL specimens per patient and will not be repeated within 7 days. This assay should not be used as a test of cure, as the presence of nucleic acids can persist even when infections have been adequately treated.
Revised
5/11/23
Initials
SA