Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Synonyms
2425D; 2425R; Hydroycalcifediol; Hydroxycalcidiol; P450CC24; Cytochrome P450 Family 24; Cytochrome P450 Subfamily XXIV; Vitamin D 24-Hydroxylase; Dihydrocholesterol; 2425OHD; CYP24A1; Calciferol; Ergocalciferol; Calcidiol; Cholecalciferol; Colecalciferol; Vitamin D Assay; CYP24
Specimen Type
Serum
Performing Lab
Mayo Medical Lab
Epic ID
Other Reference Test
Lab Test Days
Tuesday and Thursday
Standard TAT
2-6 Days
Collection Information
Specimen Required
2.5-6 mL Blood
Container Type
Red Top (No Additive)
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK/Ambient-OK
Specimen Stability
Room Temp: 7 Days; Refrigerated: 14 Days (Preferred); Frozen: 30 Days
Rejection Criteria
Gross lipemia
Test Volume Required
3 mL Serum
Minimum Test Volume
1.1 mL Serum
Processing Instructions
Separate serum from cells within 2 hours of collections.
Included Tests
25-Hyrdroxy D2, 25HDN:24,25 Dihydroxy VitD Ratio, 25-Hydroxy D3, 25-Hydroxy D Total and 24,25-Dihydroxy VitD Total.
CPT Codes
82306, 82542
Revised
12-01-18
Initials
SL