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Lab Test Dictionary

25-Hydroyvitamin D:24,25-Dihydroxyvitamin D Ration, Serum (2425D)


Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)


2425D; 2425R; Hydroycalcifediol; Hydroxycalcidiol; P450CC24; Cytochrome P450 Family 24; Cytochrome P450 Subfamily XXIV; Vitamin D 24-Hydroxylase; Dihydrocholesterol; 2425OHD; CYP24A1; Calciferol; Ergocalciferol; Calcidiol; Cholecalciferol; Colecalciferol; Vitamin D Assay; CYP24

Specimen Type


Performing Lab

Mayo Medical Lab

Epic ID

Other Reference Test

Lab Test Days

Tuesday and Thursday

Standard TAT

2-6 Days

Collection Information

Specimen Required

2.5-6 mL Blood

Container Type

Red Top (No Additive)

Processing Information - Lab Use Only

Transport Temperature


Specimen Stability

Room Temp: 7 Days; Refrigerated: 14 Days (Preferred); Frozen: 30 Days

Rejection Criteria

Gross lipemia

Test Volume Required

3 mL Serum

Minimum Test Volume

1.1 mL Serum

Processing Instructions

Separate serum from cells within 2 hours of collections.

Included Tests

25-Hyrdroxy D2, 25HDN:24,25 Dihydroxy VitD Ratio, 25-Hydroxy D3, 25-Hydroxy D Total and 24,25-Dihydroxy VitD Total.

CPT Codes

82306, 82542