Synonyms
Ped Cell Deoxy ATP Level, RBC Deoxy ATP Level, Adenosine Deaminase, PEG-ADA; Adagen Level
Specimen Type
Whole Blood
Performing Lab
Duke Research-Dr. Hershfield
Epic ID
Other Reference Test
Collection Information
Specimen Required
2 mL Whole Blood
Container Type
Dark Green (Sodium Heparin)
Collection Instructions
Whole blood in an EDTA lavender-top tube is also acceptable. Collect only Monday-Thursday only. Ship same day as collected. If patient has received RBC transfusion within 2 months, write "TRANSFUSED" on the request for testing. Best to draw in MORNING to avoid washed cell procedure.
Processing Information - Lab Use Only
Transport Temperature
Ambient-Only
Specimen Stability
Room Temp: Whole Blood Only; Refrigerated: Unacceptable; Frozen: Plasma and RBC Only
Test Volume Required
2 mL Whole Blood
Minimum Test Volume
2 mL Whole Blood
Processing Instructions
If there is a delay in shipping, blood must be separated into plasma and washed, packed RBC. Try to maintain sample at colde temperature during separation and washing of RBC (not critical). Plasma: Transfer 0.2-1 mL to labeled tube (microfuge tube or screw cap plastic freezer vial). Red Cells: Wash with normal saline or PBS. Completley remove supernatant (important). Store the washed RBC and the plasma frozen. Once frozen, do not thaw. Then, ship both the plasma and washed, packed RBC on dry ice.
Comments
Research only, no charge. Contact Dr. Michael Hershfield at Duke University for questions regarding testing and requirements at (919) 684-4184.
CPT Codes
Research only, no charge.
Revised
09-14-15
Initials
SL