Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Specimen Type
See Collection Instructions
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB4042
Lab Test Days
Daily
Standard TAT
2-4 Days
Collection Information
Specimen Required
0.35-1 mL Bronchial lavage/wash or Sputum or CSF
Container Type
Sterile Container
Collection Instructions
Throat swab or Nasopharyngeal swab collected in VCM medium (green cap) tube or equivalent (UTM) is also acceptable. Nasopharyngeal and Throat swab in M4, VCM (UTM) Media (Use only sterile Dacron or rayon swabs.) Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap. Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container. Bronchial Lavage: Collect in a sterile, leak-proof plastic container.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: 48 Hours; Refrigerated: 7 Days; Frozen: 30 Days
Rejection Criteria
Calcium alginate swabs; AVANTIK VTM
Test Volume Required
1 mL Bronchial lavage/wash or Sputum
Minimum Test Volume
0.35 mL Bronchial lavage/wash or Sputum
Processing Instructions
Indicate source of specimen in comments, on specimen and on batch sheet.
Included Tests
Mycoplasma pneumoniae, Legionella, and Chlamydophila pneumoniae DNA, Qualitative, RT-PCR.
CPT Codes
87486, 87541, 87581, 87798
Revised
11/10/23
Initials
SA