Seattle Cancer Care Alliance Pharmacokinetics Lab
1-3 mL Blood
Dark Green (Sodium Heparin)
Label blood tubes with the actual clock time drawn. For IV busulfan, draw times are related to the starting time of a 2 hour (120 minute) infusion. For instance, the 135 minute draw would be done 15 minutes after the end of infusion, the 150 minute draw would be done 30 minutes after the end of infusion, the 3 hour drawn would be done 1 hour after the end of infusion, etc. Immediately after specimen collection place on wet ice slurry and refrigerated at 4 C. Busulfan degrades rapidly at room temp. Submit each specimen to lab on wet ice immediately after collection.
1.5 mL Plasma
0.5 mL Plasma
Centrifuge samples as soon as possible at 4 C. Separate the plasma from RBC's. Store the plasma in plastic storage tubes immediately at -20 C. Labels must have patient name, MRN, date and actual clock time of draw. Samples must be shipped on a minimum of 5 kg of dry ice the day they were drawn using an overnight carrier. Include the fully completed Busulfan Pharmacokinetics requisition with the samples. Saturday deliveries require "Saturday Delivery" specified on the outside package.