Real-Time Polymerase Chain Reaction (RT-PCR)
EV PCR; EV D68; Coxsackie Virus PCR; Echovirus PCR
See Collection Instructions
Quest Diagnostics San Juan Capistrano
1-2 mL Blood
Plasma collected in an EDTA (lavender-top) or ACD Solution A/B (yellow-top) tube; or serum collected in a (red-top) tube; or 0.5-1 g stool collected in a sterile leak-proof container; or 0.3-0.5 mL rectal, throat, lesion or nasopharyngeal swab collected in VCM medium (green-cap) or equivalent (UTM) is also acceptable.
Room Temp=48 Hours; Refrigerated=7 Days; Frozen=30 Days
0.7 mL Serum or Plasma
0.3 mL Serum or Plasma
Separate serum or plasma from cells within 2 hours of collection. Avoid repeated freezing and thawing of specimens. Indicate source in comments, on specimen and on batch sheet.
This test does not differentiate among the EV serotypes. For CSF, refer to the in house Enterovirus PCR, Qualitative.