Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Synonyms
EV PCR; EV D68; Coxsackie Virus PCR; Echovirus PCR
Specimen Type
See Collection Instructions
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB01201
Lab Test Days
Monday-Saturday
Standard TAT
1-2 Days
Collection Information
Specimen Required
1-2 mL Blood
Container Type
See Below
Collection Instructions
Plasma collected in an EDTA (lavender-top) or ACD Solution A/B (yellow-top) tube; or serum collected in a (red-top) tube; or 0.5-1 g stool collected in a sterile leak-proof container; or 0.3-0.5 mL rectal, throat, lesion or nasopharyngeal swab collected in VCM medium (green-cap) or equivalent (UTM) is also acceptable.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp=48 Hours; Refrigerated=7 Days; Frozen=30 Days
Test Volume Required
0.7 mL Serum or Plasma
Minimum Test Volume
0.3 mL Serum or Plasma
Processing Instructions
Separate serum or plasma from cells within 2 hours of collection. Avoid repeated freezing and thawing of specimens. Indicate source in comments, on specimen and on batch sheet.
Comments
This test does not differentiate among the EV serotypes. For CSF, refer to the in house Enterovirus PCR, Qualitative.
CPT Codes
87498
Revised
09-27-22