Methodology
Chromogenic Assay
Synonyms
Factor 8
Specimen Type
Plasma
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
Other Reference Test
Lab Test Days
Wednesday and Saturday
Standard TAT
1-5 Days
Collection Information
Specimen Required
(one) 1.8 mL or (one) 2.7 mL Blood
Container Type
Light Blue (Sodium Citrate)
Collection Instructions
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Specimen Stability
Room Temp: 8 Hours; Refrigerated: 8 Hours; Frozen: 21 Days
Rejection Criteria
Received room temp; Received refrigerated
Test Volume Required
1 mL Platelet-Poor Plasma
Minimum Test Volume
0.5 mL Platelet-Poor Plasma
Processing Instructions
Platelet Poor Plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and send frozen.
CPT Codes
85240
Revised
12-01-19
Initials
SL