Methodology
Clot Detection
Synonyms
Hemophilia A; Procoagulant Factor 8; Hemophiliac Factor; Antihemophilic Factor; Factor VIII Functional
Specimen Type
Plasma
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB00967
Lab Test Days
Sunday-Friday
Standard TAT
2-4 Days
Collection Information
Specimen Required
(one) 1.8 mL or (one) 2.7 mL Blood
Container Type
Light Blue (Sodium Citrate)
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Specimen Stability
Room Temp: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 Year
Rejection Criteria
Hemolysis; Thawed
Test Volume Required
1 mL Platelet-Poor Plasma
Minimum Test Volume
0.5 mL Platelet-Poor Plasma
Processing Instructions
Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plaasma must be free of platelets (10,000/mcL). Freeze immediately and send frozen.
Comments
Storage of whole blood at refrigerated temp prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.
CPT Codes
85240
Revised
10-27-19
Initials
SL