Methodology
Flow Cytometry
Synonyms
T- and B-Cell Quantitation and Lymphocyte Proliferation to Mitogens Panel; Infant (<1 year old) Immunology Panel
Specimen Type
Whole Blood
Performing Lab
Mayo Medical Lab
Epic ID
LAB00220
Lab Test Days
Monday-Thursday
Standard TAT
TBBS: 1-4 Days; LPMGF: 8-11 Days
Collection Information
Specimen Required
1-3 mL EDTA (lavender-top) Whole Blood ***and*** 1-3 mL Sodium Heparin (green-top) Whole Blood
Container Type
See Below
Collection Instructions
Draw MONDAY-THURSDAY between 0900-1300 ONLY, no weekends or holidays. Send to lab immediately after collection. Specimens must arrive at Mayo within 24 Hours of collection. If WBC is low, collect more volume of blood.
Processing Information - Lab Use Only
Transport Temperature
Ambient-Only
Specimen Stability
Room Temp: 48 Hours for Sodium Heparin Whole Blood, 52 Hours EDTA for Whole Blood; Refrigerated: Unacceptable; Frozen: Unacceptable
Rejection Criteria
Gross hemolysis; Gross lipemia; Specimens in aliquot tubes (not in original vacutainers)
Test Volume Required
3 mL EDTA (lavender-top) Whole Blood ***and*** 3 mL Sodium Heparin (green-top) Whole Blood
Minimum Test Volume
1 mL EDTA (lavender-top) Whole Blood ***and*** 1 mL Sodium Heparin (green-top) Whole Blood
Included Tests
TBBS: QN Lymphocyte Subsets (T, B and NK (CD45 Total Lymph Count, CD3 (T Cells), CD4 (T Cells); CD8 (T-Cells), CD19 (B-Cells), CD16 CD56 (NK Cells), 4/8 Ratio); LPMGF: Lymphocyte Proliferation, Mitogens (Max Prolif of PWM as CD45/CD3/CD19 and PHA as CD45/CD3).
Comments
Not genetic, auth not needed
CPT Codes
86355, 86357, 86359, 86360, 86353
Revised
10-14-18
Initials
SL