Specimen Type
CSF
Performing Lab
CHLA
Epic ID
Other Reference Test
Lab Test Days
Monday-Friday
Collection Information
Specimen Required
1-2 mL CSF
Container Type
Sterile Container
Collection Instructions
Must received and processed in the lab within 1 hour of collection.
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Rejection Criteria
Unspun CSF
Test Volume Required
2 mL CSF Supernatant
Minimum Test Volume
1 mL CSF Supernatant
Processing Instructions
1. Spin CSF (note: it may be necessary to transfer CSF to a tube to spin if necessary) in a pre-cooled centrifuge (located in the research lab) at 4 C, 3000 x g for 10 minutes. May need to adjust settings on research refrigerated centrifuge. Please return to original settings when finished. 2. Transfer supernatant to a new , labeled tube leaving behind approximately 0.2 mL and making sure not to disturb pellet. Freeze supernatant at -20 or -80 until ready to ship. Store the 0.2 mL (pellet) left behind in the referral testing processing rack and write pellet on tube. Complete a CHLA LBSeq4Kids requisition, clinical indication and physician signature is required. Ship sample frozen preferred same day or next business day Monday-Thursday only, no Fridays, weekends or holidays.
Comments
Insurance authorization required prior to collection for Outpatients. Ok to send on Inpatients.
CPT Codes
81479
Revised
8/14/25
Initials
SA