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Lab Test Dictionary

Legionella Pneumophila Antigen, DFA (34475)


Direct Immunofluorescence Assay (DFA)

Specimen Type

See Collection Instructions

Performing Lab

Quest Diagnostics San Juan Capistrano

Epic ID


Lab Test Days


Standard TAT

1-2 Days

Collection Information

Specimen Required

1 g Lung tissue or Fresh (unfixed) tissue, 2-3 mL Bronchial alveolar lavage/wash (BAL), Sputum, Pleural fluid, Nasopharyngeal lavage/wash or Tracheal lavage/wash

Container Type

Sterile Container

Collection Instructions

Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container. Tissue: Add small amount of sterile, non-bacteriostatic, distilled water to prevent drying, if necessary. Do not add saline because it may be inhibitory.

Processing Information - Lab Use Only

Transport Temperature


Specimen Stability

Room Temp: Unacceptable; Refrigerated: 7 Days; Frozen: Unacceptable

Rejection Criteria

Expired transport devices; Environmental sources; Received in viral transport media

Test Volume Required

1 g or 3 mL

Minimum Test Volume

1 g or 2 mL

Processing Instructions

Indicate source in comments, on specimen and on batch sheet.

CPT Codes