Methodology
Flow Cytometry (FC)
Synonyms
Flow
Specimen Type
See Collection Instructions
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB01265
Lab Test Days
Daily
Standard TAT
3-4 Days
Collection Information
Specimen Required
3-5 mL Whole Blood or 1-4 mL Bone Marrow
Container Type
Dark Green (Sodium Heparin)
Collection Instructions
Bone Marrow or Whole Blood collected in ACD Solution A (yellow-top) or EDTA (lavender-top) tube; Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (Tissue is disaggregated into single cells so that a miniumum of 50,000 cells of interest are harvested.) Ship tissue in sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately. Body fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. Minimum of 50,000 cells of interest in total volume of fluid. Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately; Body Fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. ( A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately is also acceptable. A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood. Do not freeze and do not place in fixative. Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Ambient-OK
Specimen Stability
Room Temp: 72 Hours (May extend to 5 Days depending on cell viability); Refrigerated: Not Established; Frozen: Not Established
Test Volume Required
4 mL Bone Marrow or 5 mL Whole Blood
Minimum Test Volume
1 mL Bone Marrow or 3 mL Whole Blood
Processing Instructions
Indicate source in comments, on specimen and on batch sheet. ALL specimens for this test need to be packaged and submitted to Quest in a Hematopathology Specimen Transport box.
Included Tests
Initial markers to be evaluated are CD2-5, CD7-8, CD10-11, CD13, CD19-20, CD23, CD33-34, CD38, CD56, CD64, CD117, HLA-DR, sKAPPA, sLAMBDA, and CD45 (for gating purposes).
Comments
Insurance authorization required prior to draw for Outpatients. Ok to send on Inpatients. No special forms needed.
CPT Codes
88184, 88185 (x21), 88189
Revised
03-31-21