Methodology
Clot Detection
Specimen Type
Plasma
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB01401
Lab Test Days
Monday-Saturday
Standard TAT
1-2 Days
Collection Information
Specimen Required
(three-four) 1.8 mL or (two-three) 2.7 mL Blood
Container Type
Light Blue (Sodium Citrate)
Processing Information - Lab Use Only
Transport Temperature
Frozen-Only
Specimen Stability
Room Temp: Unacceptable; Refrigerated: Unacceptable; Frozen: 90 Days
Rejection Criteria
Hemolysis; Received thawed
Test Volume Required
3 mL Platelet-Poor Plasma
Minimum Test Volume
2 mL Platelet-Poor Plasma
Processing Instructions
Platelet Poor Plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and send frozen.
Included Tests
PTT-LA and dRVVT with Reflex Confirmations. If PTT-LA is >40 seconds, Hexagonal Phase Confirmation will be performed. If Hexagonal Phase Confirmation is positive or weakly positive, Thrombin Clotting Time will be performed. If dRVVT is >45 seconds, dRVVT Confirmation will be performed. If dRVVT Confirmation is positive, dRVVT 1:1 Mixing Study will be performed.
CPT Codes
85613, 85730; 85598; 85670; 85597; 85613
Revised
11-10-19
Initials
SL