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Lab Test Dictionary

Lymphocyte Subsets (2901300)


Flow Cytometry


T, B & NK Cell Enumeration; Lymphocyte Subpopulations; CD4:CD8 testing; T Cell Subpopulation

Specimen Type

Whole Blood

Performing Lab

Cincinnati Children's Diagnostic Immunology

Epic ID


Lab Test Days


Standard TAT

2 Days

Collection Information

Specimen Required

0.5-1 mL Whole Blood

Container Type

Lavender (EDTA)

Collection Instructions

Collect Monday-Thursday 0800-1300 ONLY, no weekends or holidays. Whole blood collected in a sodium heparin (green-top) tube is also acceptable. A concurrent CBC and differential are required in order to report absolute counts.

Processing Information - Lab Use Only

Transport Temperature


Specimen Stability

Room Temp: 24 Hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Rejection Criteria

Frozen; Clotted; Hemolyzed; Centrifuged specimens; Specimen collected in an unacceptable anticoagulant

Test Volume Required

1 mL Whole Blood

Minimum Test Volume

0.5 mL Whole Blood

Processing Instructions

Complete Cincinnati Diagnostic Immunology requisition, concurrent CBC/Differential and submit with specimen room temp. Ship Monday-Thursday ONLY, no weekends or holidays.

Included Tests

% and absolute count for the lymphocyte subpopulations: Total T cells (CD3), T helper cells (CD4), T cytotoxic cells (CD8), B cells (CD19), and NK cells (CD16/CD65); and CD4:CD8 ratio.

CPT Codes

86360, 86359, 86355, 86357