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Lab Test Dictionary

Pediatric Cellular Immune Panel (Child >1 year old) (TBBS, LPAGF and LPMGF)

Methodology

Flow Cytometry

Synonyms

T- and B-Cell Quantitation and Lymphocyte Proliferation to Antigens and Mitogens Panel; T-Cell Immune Competence Panel

Specimen Type

Whole Blood

Performing Lab

Mayo Medical Lab

Epic ID

LAB00260

Lab Test Days

Monday-Thursday

Standard TAT

TBBS: 1-4 Days;LPAFG: 11-14 Days;LPMGF: 8-11 Days

Collection Information

Specimen Required

1-3 mL EDTA (lavender-top) Whole Blood ***and*** (two) 3-10 mL Sodium Heparin (green-top)

Container Type

See Below

Collection Instructions

Draw MONDAY-THURSDAY between 0900-1300 ONLY, no weekends or holidays. Send to lab immediately after collection. Specimens must arrive at Mayo within 24 Hours of collection. If WBC is low, collect more volume of blood.

Processing Information - Lab Use Only

Transport Temperature

Ambient-Only

Specimen Stability

Room Temp: 48 Hours for Sodium Heparin Whole Blood, 52 Hours for EDTA Whole Blood; Refrigerated: Unacceptable; Frozen: Unacceptable

Rejection Criteria

Gross hemolysis; Gross lipemia; Specimens in aliquot tubes (not in original Vacutainers)

Test Volume Required

3 mL EDTA (lavender-top) Whole Blood ***and*** (two) 10 mL Sodium Heparin (green-top)

Minimum Test Volume

1 mL EDTA (lavender-top) Whole Blood ***and*** (two) 3 mL Sodium Heparin (green-top)

Included Tests

TBBS: QN Lymphocyte Subsets (T, B and NK (CD45 Total Lymph Count, CD3 (T Cells), CD4 (T Cells); CD8 (T-Cells), CD19 (B-Cells), CD16 CD56 (NK Cells), 4/8 Ratio); LPAFG: Lymphocyte Proliferation, Antigens (Max Prolif of CA and TT as CD45/CD3); LPMGF: Lymphocyte Proliferation, Mitogens (Max Prolif of PWM as CD45/CD3/CD19 and PHA as CD45/CD3).