Methodology
Direct Immunofluorescence Assay (DFA)
Synonyms
PCP; Pneumocystis jaroveci
Specimen Type
Respiratory Specimen
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
Other Reference Test
Lab Test Days
Monday-Saturday
Standard TAT
1-2 Days
Collection Information
Specimen Required
2-3 mL Bronchial lavage/wash, sputum, or bronchial alveolar lavage
Container Type
Sterile Container
Collection Instructions
Bronchial brushings collected on 2 acetone fixed slides is also acceptable.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK/Ambient-OK
Specimen Stability
Room Temp: Unacceptable (7 Days for Slides); Refrigerated: 7 Days; Frozen: Unacceptable (30 Days for Slides)
Rejection Criteria
Broken slides; Slides fixed in formalin; Slides fixed in ethanol; Unfixed slides
Test Volume Required
3 mL
Minimum Test Volume
2 mL
CPT Codes
87015, 87281
Revised
09-27-22
© 2023 Rady Children's Hospital–San Diego