Methodology
Flow Cytometry
Synonyms
T helper 1; Hyper IgE Syndrome (Th17)
Specimen Type
Whole Blood
Performing Lab
Cincinnati Children's Diagnostic Immunology
Epic ID
Other Reference Test
Lab Test Days
Monday-Friday
Standard TAT
2 Days
Collection Information
Specimen Required
2-4 mL Whole Blood
Container Type
Sodium Heparin (Dark Green-Top) Tube
Collection Instructions
Collect Monday-Thursday 0800-1300 only, no Fridays, weekends or holidays.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-ONLY
Specimen Stability
Room Temp: 24 Hours (preferred); Refrigerated: Unacceptable; Frozen: Unacceptable
Test Volume Required
4 mL Whole Blood
Minimum Test Volume
2 mL Whole Blood
Processing Instructions
Do not spin. Complete Cincinnati Diagnostic Immunology requisition and submit with specimen room temp. Ship Monday-Thursday only, no Fridays, weekends or holidays. Do not spin. Sample must be received within 24 hours of collection.
Included Tests
Cell Surface expression of CCR6+CD45RA- and intracellular expression of IL17A+ on CD4+ Lymphocytes, Population of Th17 cells among Total CD4+ T cells
Comments
Insurance authorization is required prior to draw for Outpatients. Ok to send on Inpatients.
CPT Codes
88184, 88185 (x4), 88187
Revised
6/1/26
Initials
IM