AFB; Acid Fast Bacili; Mycobacteria; Mycobacterial; MTB; TB,
See Collection Instructions
Quest forwards to National Jewish (TSO)
Other Reference Test
24 Hours-8 Weeks
Monday-Saturday
See Below
This is the same test as Quest test code 4554 AFB Smear and Culture unless requested for Cystic Fibrosis protocol. Do not duplicate tests. Sputum: >5 mL of unprocessed (raw) sputum. Collect the first early morning expectoration in a sterile 50 mL polypropylene centrifuge tube capable of withstanding 3,000 x g (i.e, Falcon, Greiner, etc). Body Fluids or CSF: As much body fluid as possible is aseptically collected by aspiration or during a surgical procedure. CSF should be maximum volume obtained, ideally at least 2 mL. Gastric Aspirates: Must be neutralized (pH 7) with sodium carbonate if transport time exceeds four hours from collection. Transfer 5-10 mL to a sterile container. Tissue: =10 g or as much as possible with a biopsy.Transfer to a sterile container (without formalin or preservatives). Urine: = 40 mL to a sterile container. Stool: = 1 g in sterile, wax-free disposable container. Blood: =5 mL adult; =1 mL child. If blood has to be transported before inoculation of the medium, sodium polyanethol sulfate (SPS), heparin, or citrate may be used as anticoagulants.
Refrigerate-Only
Room Temp=Unacceptable; Refrigerated=Not Established; Frozen=Unacceptable
Indicate source in comments, on specimen and on batch sheet. Requires TSO form and completed NJH Mycobacteriology requisition submitted with specimen.0
National Jewish test code: AFB1. Requires TSO form and completed NJH Mycobacteriology requisition submitted with specimen. This is the same test as Quest test code 4554 AFB Smear and Culture unless requested for Cystic Fibrosis protocol. Do not duplicate tests.
87015, 87116, 87206, 87556
04-06-20
SL