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Lab Test Dictionary

AFB Smear & Culture (Clinical Specimen Only ( NAAT) (AFB1) (Special Request Only)

Synonyms

AFB; Acid Fast Bacili; Mycobacteria; Mycobacterial; MTB; TB,

Specimen Type

See Collection Instructions

Performing Lab

Quest forwards to National Jewish (TSO)

Epic ID

Other Reference Test

Lab Test Days

24 Hours-8 Weeks

Standard TAT

Monday-Saturday

Collection Information

Container Type

See Below

Collection Instructions

This is the same test as Quest test code 4554 AFB Smear and Culture unless requested for Cystic Fibrosis protocol. Do not duplicate tests. Sputum: >5 mL of unprocessed (raw) sputum. Collect the first early morning expectoration in a sterile 50 mL polypropylene centrifuge tube capable of withstanding 3,000 x g (i.e, Falcon, Greiner, etc). Body Fluids or CSF: As much body fluid as possible is aseptically collected by aspiration or during a surgical procedure. CSF should be maximum volume obtained, ideally at least 2 mL. Gastric Aspirates: Must be neutralized (pH 7) with sodium carbonate if transport time exceeds four hours from collection. Transfer 5-10 mL to a sterile container. Tissue: =10 g or as much as possible with a biopsy.Transfer to a sterile container (without formalin or preservatives). Urine: = 40 mL to a sterile container. Stool: = 1 g in sterile, wax-free disposable container. Blood: =5 mL adult; =1 mL child. If blood has to be transported before inoculation of the medium, sodium polyanethol sulfate (SPS), heparin, or citrate may be used as anticoagulants.

Processing Information - Lab Use Only

Transport Temperature

Refrigerate-Only

Specimen Stability

Room Temp=Unacceptable; Refrigerated=Not Established; Frozen=Unacceptable

Processing Instructions

Indicate source in comments, on specimen and on batch sheet. Requires TSO form and completed NJH Mycobacteriology requisition submitted with specimen.0

Comments

National Jewish test code: AFB1. Requires TSO form and completed NJH Mycobacteriology requisition submitted with specimen. This is the same test as Quest test code 4554 AFB Smear and Culture unless requested for Cystic Fibrosis protocol. Do not duplicate tests.