Lab Test Dictionary

Anti-Nuclear Antibodies (ANA), IgG by ELISA w/Reflex and ENA Confirmation (0050317)

Methodology

Qualitative Enzyme-Linked Immunosorbent Assay; Semi-Quantitative Indirect Fluorescent Antibody; Semi-Quantitative Multiplex Bead Assay; Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Synonyms

ANA Reflexive Profile; FANA; Fluorescent Antinuclear Antibody; ANA, dsDNA, RNP, Smith, SSA, SSB IgG; Antinuclear Antibody; ANA IgG panel; ANA IgG reflex; ANA panel; ANA reflex

Specimen Type

Serum

Performing Lab

ARUP

Epic ID

Other Reference Test

Lab Test Days

Daily

Standard TAT

1-5 Days

Collection Information

Specimen Required

1.5-2 mL Blood

Container Type

Gold (SST)

Processing Information - Lab Use Only

Transport Temperature

Refrigerate-OK/Frozen-OK

Specimen Stability

Room Temp: 48 Hours; Refrigerated: 2 Weeks (Preferred); Frozen: 1 Year (avoid repeated freezethaw cycles)

Rejection Criteria

Non-serum specimens; Contaminated; Grossly hemolyzed; Heat-activated; Severely lipemic; Specimens or inclusion of fibrin clots

Test Volume Required

1 mL Serum

Minimum Test Volume

0.6 mL Serum

Processing Instructions

Separate serum from cells within 2 hours of collection. Complete an ARUP requisition and submit with specimen refrigerated. Send specimen with ARUP Courier Monday-Friday at 1300.

Included Tests

Anti-Nuclear Antibodies (ANA), IgG by ELISA; Nuclear Antibody (ANA) by IFA, IgG; Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA; RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 and 60 (Ro) (ENA) Antibodies, IgG; SSB (La) (ENA) Antibody, IgG.