Coupled Enzymatic Assay
Serum or Plasma
Next Molecular Analytics
1-2 mL Blood
After collection, invert the tube gently 4 times to mix the blood with the EDTA ( no longer than one minute after drawing the sample). Place tube on ice immediately after mixing (no longer than one minute after gently mixing the blood with the EDTA). Plasma collected in lithium or sodium heparin (green-top) tube is also acceptable. Serum collected in a (red-top) or SST tube is also acceptable but DOES NOT need to be placed on ice after collection.
Room Temp=Unacceptable; Refrigerated=7 Days (Preferred); Frozen=Indefinite
1 mL Serum or Plasma
0.3 mL Serum or Plasma
Plasma: Centrifuge the tube immediately (within 15 minutes after blood collection), in a refrigerated centrifuge at a speed of approximately 3,000 rpm for 5 minutes. Transfer plasma within 5 minutes after centrifugation. Serum: Allow blood to clot at room temperature for a minimum of 30, but not longer than 60 minutes. Centrifuge the tube at approximately 3,000 rpm for 15 minutes. Avoid contaminating serum with red blood cells. Complete a Next Molecular Analytics requisition, include ICD-10 code, dosage, and drug administered, and submit with specimen refrigerated Monday-Thursday only, no weekends or holidays.