Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
P210 BCR-ABL transcript, t(9;22); P190 BCR-ABL transcript; Philadelphia Chromosome
Whole Blood or Bone Marrow
Quest Diagnostics San Juan Capistrano
4-6 mL Whole Blood or 2-3 mL Bone Marrow
Bone marrow collected in a sodium heparin (green-top) or ACD Solution B (yellow-top) tube is also acceptable.
Room Temp=72 Hours; Refrigerated=72 Hours; Frozen=Unacceptable
6 mL whole blood or 3 mL bone marrow
4 mL Whole Blood or 2 mL Bone Marrow
Indicate specimen source in comments, on specimen and batch sheet.
If patient has not been previously documented as positive, both P190 and P210 will be performed. If patient has previously been documented with P190 transcript expression, only P190 BCR-ABL1 will be performed. If patient has previously been documented with P210 transcript expression, only P210 BCR-ABL1 will be performed.
Insurance authorization required prior to draw for Outpatients. Ok to send on Inpatients.