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Lab Test Dictionary

Bordetella Pertussis and Parapertussis, Qualitative RT-PCR, VTM

Methodology

Real Time-PCR


Synonyms

Whooping cough; B. pertussis; B. parapertussis

Specimen Type

N/P Swab

Performing Lab

RCHSD MDL (PCR)


Epic ID

LAB952

Lab Test Days

Daily

Standard TAT

48 Hours


Collection Information

Container Type

Viral Media

Collection Instructions

1. Carefully insert a flexible-wire swab through one nostril into the posterior nasopharynx and rotate the swab. Keep the swab near the septum and floor of the nose. 2. Repeat the collection steps using the same swab for the other nostril. 3. Place the entire swab into the transport tube, immersing the tip into the fluid. 4. Carefully cap the tube to prevent leakage. Label with patient’s first and last name and DOB or other unique identifier such as medical record number. 5. Remote locations: Store on ice or refrigerate. Transport on cold packs. 6. Results are available within 48 hours of specimen receipt.


Processing Information - Lab Use Only

Transport Temperature

Refrigerate-OK/Frozen-OK

Specimen Stability

Room Temp: Unacceptable; Refrigerated: 7 Days; Frozen: >7 Days

Rejection Criteria

Unlabeled or incompletely labeled specimens; Specimens with signs of leakage; Specimen with calcium alginate swabs; Nasal/Anterior Nares collections

Test Volume Required

3 mL

Minimum Test Volume

3 mL

Processing Instructions

Do not open bag containing specimen outside of the Biosafety hood. Place VTM in -80 freezer MDL (PCR) bin ASAP.


Included Tests

Bordetella pertussis and Bordetella parapertussis.


CPT Codes

87798 (x2)


Revised

1/4/24

Initials

SA