Methodology
Real Time-PCR
Synonyms
Whooping cough; B. pertussis; B. parapertussis
Specimen Type
N/P Swab
Performing Lab
RCHSD MDL (PCR)
Epic ID
LAB952
Lab Test Days
Daily (0600-1300)
Standard TAT
1-2 Days
Collection Information
Container Type
Viral Media
Collection Instructions
1. Carefully insert a flexible-wire swab through one nostril into the posterior nasopharynx and rotate the swab. Keep the swab near the septum and floor of the nose. 2. Repeat the collection steps using the same swab for the other nostril. 3. Place the entire swab into the transport tube, immersing the tip into the fluid. 4. Carefully cap the tube to prevent leakage. Label with patient’s first and last name and DOB or other unique identifier such as medical record number. 5. Remote locations: Store on ice or refrigerate. Transport on cold packs. 6. Results are available within 48 hours of specimen receipt.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: Unacceptable; Refrigerated: 7 Days; Frozen: >7 Days
Rejection Criteria
Leaked specimen, Calcium alginate swab; Nasal/Anterior Nares collections
Test Volume Required
3 mL
Minimum Test Volume
3 mL
Processing Instructions
Do not open bag containing specimen outside of the Biosafety hood. Place VTM in -80 freezer MDL (PCR) bin ASAP.
Included Tests
Bordetella pertussis and Bordetella parapertussis.
CPT Codes
87798 (x2)
Revised
9/11/23
Initials
SA