Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Synonyms
Nighttime Salivary Cortisol; Salivary Cortisol; Salivary Cushings; Salivary Hydrocortisone
Specimen Type
See Collection Instructions
Performing Lab
Mayo Medical Lab
Epic ID
Other Reference Test
Lab Test Days
Monday, Wednesday, Friday; 4 P.M.
Standard TAT
2-4 Days
Collection Information
Specimen Required
0.6-1.5 Saliva collected in a SARSTEDT Salivette Collection Kit
Container Type
See Below
Collection Instructions
Call lab for SARSTEDT Salivette Collection Kit located in the send out cupboard. Patient Preparation: 1. Do not brush teeth before collecting specimen. 2. Do not eat or drink for 15 minutes prior to specimen collection. Collection Instructions: 1. Collect specimen between 11 p.m. and midnight, and record collection time. 2. To use the Salivette: a. Remove top cap of container to expose swab. b. Place swab directly into mouth by tipping container so swab falls into mouth. Do not touch swab with fingers. c. Keep swab in mouth for approximately 2 minutes. Roll swab in mouth, do not chew swab. d. Place swab back into its container without touching, and replace the cap. 3. Record collection time, and send appropriately labeled Salivette to laboratory.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK/Ambient-OK
Specimen Stability
Room Temp: 7 Days; Refrigerated: 7 Days (Preferred); Frozen: 60 Days
Test Volume Required
1.5 mL Saliva
Minimum Test Volume
0.6 mL Saliva
Processing Instructions
Order in Mayolink and send with Mayo courier Monday-Friday.
Comments
Reference values are also available for 8 A.M. (7 A.M.-9 A.M.) and 4 P.M. (3 P.M.-5 P.M.) collections, however, 11 P.M. to midnight collection is preferred.
CPT Codes
82533
Revised
06-17-19
Initials
SL