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Lab Test Dictionary

COVID-19 RNA, Qualitative RT-PCR VTM (WET SWAB)

Methodology

RT-PCR


Synonyms

SARS-COV-2; COVID, COVID19; Coronavirus

Specimen Type

Swab

Performing Lab

RCHSD Molecular Diagnostics (MDL)


Epic ID

LAB09286

Lab Test Days

Daily

Standard TAT

4-6 hours

Stat TAT

Use Rapid Liat COVID test instead


Collection Information

Specimen Required

See collection instructions

Container Type

Viral Media

Collection Instructions

1. Nasopharyngeal swabs (NPS) or nasal swabs (NS) in Copan Universal Transport Media (UTM) or BD Universal Viral Transport (UVT) or equivalent, Remel M5, Remel M6, Copan ESwab™ (Liquid Amies), Puritan® UniTranz-RT®, or saline (0.9% sodium chloride in water). 2. Bronchoalveolar lavage (BAL) undiluted or diluted 1:1 (v/v) in a mucolytic such as Remel Sputasol. 3. Nasal wash/aspirate (NW) undiluted. Use only swabs with a synthetic tip (e.g. Dacron, nylon, or rayon) and an aluminum or plastic shaft. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Do not put swab into viral transport media with less than 3 mL volume.


Processing Information - Lab Use Only

Transport Temperature

Refrigerate-OK/Frozen-OK/Ambient-OK

Specimen Stability

No limitation

Rejection Criteria

Leaked specimen, unlabelled/mislabeled specimen, specimen with calcium alginate swabs

Test Volume Required

3 mL VTM, or 1.0 mL for BAL or nasal wash

Minimum Test Volume

3 mL VTM, or 1.0 mL for BAL or nasal wash

Processing Instructions

Do not open bag containing specimen outside of the Biosafety hood.


Included Tests

Sars-CoV-2 RT-PCR


Comments

N/A


CPT Codes

87635


Revised

07-07-22

Initials

KCE