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Lab Test Dictionary

COVID-19 RNA, Qualitative RT-PCR VTM (WET SWAB)

Methodology

RT-PCR (Diasorin)


Synonyms

SARS-COV-2; COVID; COVID19; Coronavirus

Specimen Type

NP or NS Swab or Respiratory Fluid

Performing Lab

RCHSD MDL


Epic ID

LAB09286

Lab Test Days

Daily (0600-1200); Tested on SARS only Rapid test (Liat)/RP2 (ePlex) in Micro afterhours.

Standard TAT

4-6 Hours

Stat TAT

Use Rapid Liat COVID test instead


Collection Information

Specimen Required

Nasopharyngeal (NP) swabs or nasal swabs (NS) in Copan Universal Transport Media (UTM) or BD Universal Viral Transport (UVT) or equivalent, Remel M5, Remel M6, Copan ESwab (Liquid Amies), Puritan UniTranz-RT or saline (0.9% sodium chloride in water).

Container Type

Viral Media

Collection Instructions

Bronchoalveolar lavage (BAL) undiluted or diluted 1:1 (v/v) in a mucolytic such as Remel Sputasol or Nasal wash/aspirate (NW) undiluted is also acceptable. Use only swabs with a synthetic tip (e.g. Dacron, nylon, or rayon) and an aluminum or plastic shaft. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Do not put swab into viral transport media with less than 3 mL volume.


Processing Information - Lab Use Only

Transport Temperature

Refrigerate-OK/Frozen-OK/Ambient-OK

Specimen Stability

No limitation

Rejection Criteria

Leaked specimen; Unlabeled or incompletely labeled specimens; Specimen with calcium alginate swabs

Test Volume Required

3 mL VTM; or 1 mL for BAL or Nasal Wash

Minimum Test Volume

3 mL VTM; or 1 mL for BAL or Nasal Wash

Processing Instructions

Do not open bag containing specimen outside of the Biosafety hood. Receive specimen into lab and place in MDL bucket for pick up. If ordered STAT, deliver to Micro bench staff promptly for SARS only Rapid test (Liat). DO NOT place in Micro bucket.


Included Tests

Sars-CoV-2 RT-PCR


Comments

Tested on SARS only Rapid test (Liat)/RP2 (ePlex) in Micro afterhours.


CPT Codes

87635


Revised

5/17/24

Initials

SA