Methodology
Immunofluorescence Assay (IFA); Tissue Culture
Synonyms
Viral Culture; Virus Culture
Specimen Type
See Collection Instructions
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB01496
Lab Test Days
Monday-Saturday
Standard TAT
2-24 Days
Collection Information
Specimen Required
1-2 mL Body fluids, tissues, conjunctival swab or 2-3 mL Newborn urine
Container Type
Viral Media
Collection Instructions
1-2 mL CSF collected in a sterile container is also acceptable. Body fluids: For all body fluids add an equal volume of specimen to transport medium (VCM or equivalent). Newborn urine: Random urine, random clean catch urine, catheterized urine, or first void catch urine in sterile screw-capped container. Tissue and biopsy material: Sterile screw-capped container with small amount of saline, no fixative or preservative. Requested volume is as much as possible. CSF: PCR is preferable to culture.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp=Unacceptable; Refrigerated=4 Days (72 Hours for Raw (unpreserved) specimens); Frozen=30 Days (Unacceptable for Raw (unpreserved) specimens)
Rejection Criteria
Wooden shaft and calcium alginate swabs; Dry swabs; Transwabs or swabs in bacterial transport medium; DNA probe transport systems; Tissues in formalin or other fixatives; Transports for antigen detection by EIA; Received room temperature or frozen -20 C; Semen; Sputum; Whole blood; Bone marrow in EDTA (refer for PCR testing); Eye discharge; Respiratory specimens
Test Volume Required
2 mL
Minimum Test Volume
1 mL
Processing Instructions
Indicate source in comments, on specimen and on batch sheet.
Included Tests
Isolation and Identification.
Comments
If test is ordered on stool, only Quest test code 2647 Enterovirus Culture or 15082X Enterovirus, Qualiftative PCR can be done.
CPT Codes
87252
Revised
09-27-22