Methodology
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Synonyms
Cytomegalovirus, PCR; LightCycler CMV; CMV Detection by Real-Time PCR
Specimen Type
See Collection Instructions
Performing Lab
Mayo Medical Lab
Epic ID
Other Reference Test
Lab Test Days
Monday-Saturday
Standard TAT
1-3 Days
Collection Information
Container Type
See Below
Collection Instructions
Fluid (Body (ex: Pericardial Fluid, Ocular): 0.3-0.5 mL collected in a sterile container. Respiratory (Bronchial washing, Bronchoalveolar lavage, Nasopharyngeal aspirate/washing, Sputum, Tracheal aspirate): 1-1.5 mL collected in a sterile container. Genital CultureSwab (Cervic, Vagina, Urethra, Anal/Rectal, other genital sources): placed back into swab cylinder. Miscellaneous CultureSwab (Dermal, Eye, Nasal, Saliva, Throat): placed back into swab cylinder. Fresh Tissue (Brain, Colon, Kidney, Liver, Lung, etc.): in a sterile container with 1-2 mL of sterile saline or multi-microbe medium (M5). Random Urine: 0.3-1 mL collected in a sterile container. Bone marrow: 0.5 mL collected in an EDTA (lavender-top) tube.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: Unacceptable Refrigerated: 7 Days; Frozen: 7 Days
Rejection Criteria
Calcium alginate-tipped swab; Wood swab; Transport swab containing gel; Blood; Serum; Stool; Paraffin blocks; Breast milk
Processing Instructions
Indicate source in comments and on specimen.
CPT Codes
87496
Revised
06-13-18
© 2023 Rady Children's Hospital–San Diego