Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
Synonyms
DSG 3; DSG 1; Pemphigus Foliaceus, Pemphigus Vulgaris
Specimen Type
Serum
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB4136
Lab Test Days
Thursday
Standard TAT
4 Days
Collection Information
Specimen Required
1-2 mL Blood
Container Type
Red Top (No Additive)
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: 4 Days; Refrigerated: 7 Days; Frozen: 28 Days
Rejection Criteria
High levels of hemolysis; High levels of lipemia; Icetrus or microbial growth
Test Volume Required
0.3 mL Serum
Minimum Test Volume
0.1 mL Serum
Processing Instructions
Allow the blood to clot at room temp and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Do not use heat-inactivated serum as this affects test results.
Included Tests
Desmoglein 1 Antibody and Desmoglein 3 Antibody
CPT Codes
83520 (x2)
Revised
06-16-16
Initials
SL