Methodology
Flow Cytometry
Synonyms
HLA Crossmatch
Specimen Type
Whole Blood
Performing Lab
UCSD Immunogenetics
Epic ID
Other Reference Test
Lab Test Days
Monday-Friday
Standard TAT
1 Week
Collection Information
Specimen Required
8.5-20 mL (x1-4) Whole blood collected in an ACD Solution A (yellow-top) tube ***and*** (x1-2) 6-10 mL Blood collected in a (red-top) tube (For pediatric patients or patients with low WBC counts, call 858-657-5739 for special instructions)
Container Type
See Below
Collection Instructions
Collected preferably Monday-Friday before 1200. It is not recommended to collect on weekends or holidays. If WBC is low, must call UCSD Immunogenetics 858-657-5740 for an adequate draw amount. RCH Renal Transplant Coordinator must submit a completed UCSD Immunogenetics requisition with specimen to lab.
Processing Information - Lab Use Only
Transport Temperature
Ambient-Only
Specimen Stability
Room Temp: 72 Hours (7 Days for red-top tubes); Refrigerated: Unacceptable (5 Days for spun serum from red-top tube); Frozen: Unacceptable (2 Years for spun serum from red-top tube)
Rejection Criteria
Specimens >1 week old; Hemolyzed
Test Volume Required
20 mL Whole blood collected in an ACD Solution A (yellow-top) tube ***and*** 10 mL Blood collected in a (red-top) tube (For pediatric patients or patients with low WBC counts, call 858-657-5739 for special instructions)
Minimum Test Volume
8.5mL Whole blood collected in an ACD Solution A (yellow-top) tube ***and*** 6 mL Blood collected in a (red-top) tube (For pediatric patients or patients with low WBC counts, call 858-657-5739 for special instructions)
Processing Instructions
For pediatric patients or patients with low WBC counts, call 858-657-5739 for special instructions. Submit a completed UCSD Immunogenetics requisition with specimen room temp Monday-Friday before 1400. Deliver to UCSD the same day as collected if possible. If specimen is received without a completed UCSD Immunogenetics requisition, contact RCH Renal Transplant Coordinators at x223215.
CPT Codes
86825
Revised
9/4/25
Initials
SA