Methodology
Plasma-Based Next Generation Sequencing
Synonyms
Karius Digital Culture
Specimen Type
Plasma
Performing Lab
Karius
Epic ID
LAB07948
Lab Test Days
Monday-Saturday
Standard TAT
2 Days
Collection Information
Specimen Required
3-4 mL Blood
Container Type
White Plasma Preparation Tube (PPT)
Collection Instructions
White Plasma Preparation Tube (PPT) is located in send out cupboard in lab. Call lab for tube. 3-4 mL Blood collected in an EDTA (K2) (lavender-top) tube is also acceptable, if PPT is not available. Infectious Disease approval is required.
Processing Information - Lab Use Only
Specimen Stability
Room Temp: 4 Days; Refrigerated: Unacceptable; Frozen: >4 Days
Test Volume Required
2 mL Plasma
Minimum Test Volume
1.2 mL Plasma (0.7 mL minimum for Infants/no repeat testing)
Processing Instructions
Separate plasma within 6 hours of collection. Spin PPT or K2-EDTA blood tube for 10 minutes at room temp. Keep plasma in PPT. For K2-EDTA blood tube, use a sterile transfer pipette to transfer plasma to a sterile tube labeled with two identifiers. A Sterile polypropylene tube is required. Do not disturb the buffy coat: leave a small amount of plasma behind. If cells are visible in the plasma or if the buffy coat was disrupted, spin the plasma again and transfer into a new sterile tube. Infectious Disease approval is required. Create a Karius requisition. Submit completed Karius requisition and submit with specimen room temp or frozen. Ok to ship Friday for Saturday delivery. If specimen is not able to be shipped within 2-3 days of collection, freeze spun down PPT tube or EDTA (lavender-top) plasma in a sterile screw-capped vial and ship frozen.
Comments
Infectious Disease approval is required.
CPT Codes
87507, 87633, 87798 (x13)
Revised
12-01-20
Initials
SL