Methodology
Ultracentrifugation; Colorimetric; Enzymatic
Specimen Type
Serum
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
Other Reference Test
Lab Test Days
Tuesday and Friday
Standard TAT
2-4 Days
Collection Information
Specimen Required
4-8 mL Blood
Container Type
Red Top (No Additive)
Collection Instructions
Patient Preparation: The assay manufacturer, Roche, notes that N-acetylcysteine, when administered in high concentrations (such as for treatment of acetaminophen overdose), can interfere with their assay for HDL cholesterol, leading to falsely low results. The assay manufacturer Beckman Coulter advises: "N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been...determined to interfere with assays for...Cholesterol, Uric Acid" where "NAC interference may lead to falsely low results." According to Beckman Coulter, the NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hours and a third dose of 100 mg/kg administered over 16 hrs. Fasting for at least 12 hours is required.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Frozen-OK
Specimen Stability
Room Temp: 24 Hours; Refrigerated: 7 Days; Frozen: 1 Year
Test Volume Required
4 mL Serum
Minimum Test Volume
2 mL Serum
Included Tests
Cholesterol, Triglycerides, HDL, LDL and VLDL.
CPT Codes
82465, 83701, 84478
Revised
11-12-17
Initials
SL