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Lab Test Dictionary

Lymphocyte Proliferation to Antigens, Blood (LPAGF)


Flow Cytometry


Blastogenesis Antigens; Immune Competence; Lymphocyte Phythemagglutin; Lymphocyte Transformation; Pediatric Cellular Immune Panel

Specimen Type

Whole Blood

Performing Lab

Mayo Medical Lab

Epic ID

Other Reference Test

Lab Test Days


Standard TAT

11-14 Days

Collection Information

Specimen Required

3-20 mL Whole Blood (1 mL Whole Blood minimum for < 6 years old)

Container Type

Dark Green (Sodium Heparin)

Collection Instructions

Draw MONDAY-THURSDAY between 0900-1300 ONLY, no weekends or holidays. Send to lab immediately after collection. Specimens must arrive at Mayo within 24 Hours of collection. If WBC is low, collect more volume of blood.

Processing Information - Lab Use Only

Transport Temperature


Specimen Stability

Room Temp: 48 Hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Rejection Criteria

Gross hemolysis; Gross lipemia; Lithium heparin; Aliquot tube

Test Volume Required

20 mL Whole Blood

Minimum Test Volume

3 mL Whole Blood (1 mL Whole Blood minimum for < 6 years old)

Included Tests

LPAFG: Lymphocyte Proliferation, Antigens (Max Prolif of CA and TT as CD45/CD3).


This test should not be ordered for patients younger than 3 months of age unless there is a clinical history of candidiasis.

CPT Codes