Lab Test Dictionary

Lymphocyte Proliferation to Mitogens, Blood (LPMGF)

Methodology

Flow Cytometry

Synonyms

Phytohemagglutin (PHA); Blastogenesis Mitogens; Immune Competence; In vitro T-Cell Function; Lymphocyte Transformation; Pokeweed (PWM); Pediatric Cellular Immune Panel; Infant Cellular Immune Panel

Specimen Type

Whole Blood

Performing Lab

Mayo Medical Lab

Epic ID

Other Reference Test

Lab Test Days

Monday-Thursday

Standard TAT

8-11 Days

Collection Information

Specimen Required

1-10 mL Whole Blood

Container Type

Dark Green (Sodium Heparin)

Collection Instructions

Draw MONDAY-THURSDAY between 0900-1300 ONLY, no weekends or holidays. Send to lab immediately after collection. Specimens must arrive at Mayo within 24 Hours of collection. If WBC is low, collect more volume of blood.

Processing Information - Lab Use Only

Transport Temperature

Ambient-Only

Specimen Stability

Room Temp: 48 Hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Rejection Criteria

Gross hemolysis; Gross lipemia; Lithium heparin; Aliquot tube

Test Volume Required

10 mL Whole Blood

Minimum Test Volume

1 mL Whole Blood

Included Tests

LPMGF: Lymphocyte Proliferation, Mitogens (Max Prolif of PWM as CD45/CD3/CD19 and PHA as CD45/CD3).

Comments

If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test code will be reflexed and billed separately.