Methodology
Next Generation Sequencing
Synonyms
Essential Thrombocythemia; PMF; Polycythemia Vera; CALR; MPD; BCR-ABL1; ET; Polycythemia Rubra Vera; PV; JAK2; Primary Myelofibrosis; V617F
Specimen Type
Whole Blood or Bone Marrow
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB079790
Lab Test Days
Daily
Standard TAT
5-7 Days
Collection Information
Specimen Required
3-4 mL Whole Blood or 1-3 mL Bone Marrow
Container Type
EDTA (Lavender-Top) Tube
Collection Instructions
Whole blood or bone marrow collect in a sodium heparin (green-top) tube or Fixed cell pellet collected in a plastic leak-proof container is also acceptable.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-OK/Frozen-OK
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Rejection Criteria
Hemolysis
Test Volume Required
4 mL Whole Blood or 3 mL Bone Marrow
Minimum Test Volume
3 mL Whole Blood or 1 mL Bone Marrow
Processing Instructions
Cell pellets: Send fixed samples within 7 days of fixation date in a plastic leak-proof container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Included Tests
JAK2 V617F Mutation, JAK2 Exon 12 Mutation, CALR Exon 9 Mutation, and MPL Exon 10 Mutation.
Comments
Insurance authorization is required prior to draw for Outpatients. Ok to send on Inpatients.
CPT Codes
81270, 81279, 81219, 81339
Revised
2/11/25
Initials
SA