Methodology
Next Generation Sequencing (NGS)
Synonyms
MDS Somatic Mutation Panel
Specimen Type
Whole Blood or Bone Marrow
Performing Lab
UCSD Clinical Genomics (UCSD CALM)
Epic ID
LAB07969
Lab Test Days
Weekly
Standard TAT
16 Days
Collection Information
Specimen Required
1-5 mL Whole Blood or 1-3 mL Bone Marrow
Container Type
EDTA (Lavender-Top) Tube
Collection Instructions
Formalin fixed, paraffin embedded tissue (containing at least 20% tumor) is also acceptable.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-OK/Refrigerate-OK
Specimen Stability
Room Temp: 3 Days; Refrigerated: 7 Days (Holding Samples Only); Frozen: Unacceptable (Indefinitely for extract DNA only)
Rejection Criteria
Severely clotted or grossly hemolyzed specimens; Specimens that have been improperly collected, stored, or transported; Specimens collected in preservatives other than EDTA; ACD tubes are accepted, but EDTA is preferred; Serum or plasma; Specimens that have been frozen; Commingled specimens; Specimens in tubes that have been damaged or broken during transport; Specimens with insufficient volume for testing; Unlabeled or mislabeled specimens
Test Volume Required
5 mL Whole Blood or 3 mL Bone Marrow
Minimum Test Volume
1 mL Whole Blood or Bone Marrow
Processing Instructions
Complete a UCSD Clinical Genomics requisition and submit with specimen room temp only to UCSD CALM Lab. If holding to send next day or after a weekend or holiday, store sample refrigerated and ship room temp.
Comments
Insurance authorization required prior to draw for Outpatients. MJ approval for inpatients.
CPT Codes
81455
Revised
8/13/24
Initials
SA