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Lab Test Dictionary

Shiga Toxins, EIA with Reflex to Escherichia coli O157 Culture (30264)

Methodology

Broth Amplified Immunoassay with culture reflex; Direct detection without amplification or culture

Specimen Type

Stool

Performing Lab

Quest Diagnostics Valencia

Epic ID

Other Reference Test

Lab Test Days

Daily

Standard TAT

3-5 Days

Collection Information

Specimen Required

1 g or 1 mL Stool

Container Type

Cary-Blair Transport Medium

Collection Instructions

Collect in clean, dry container and then transfer a minimum of 1 gram or 1 mL into a Cary-Blair stool culture transport medium. Fluid level should reach line on vial. Frozen (-70Â° C) specimens are sub-optimal but can be tested directly without broth enhancement or reflex culture. Rectal swab in Amies or Cary-Blair stool culture transport medium, pure isolate on agar slant/tube/swab transport system or pure isolate in GN broth collected in a sterile, leak-proof container is also acceptable.

Processing Information - Lab Use Only

Transport Temperature

Refrigerate-OK/Ambient-OK

Specimen Stability

Room Temp: 4 Days; Refrigerated: 4 Days; Frozen: Unacceptable

Rejection Criteria

Swabs not in Amies or Cary-Blair stool culture transport medium; Unpreserved raw stool at room temperature; Unpreserved raw refrigerated stool >2 hours old from time of collection; Frozen specimens in Cary-Blair stool culture transport medium; Specimens in Cary-Blair >4 days old; Frozen specimens that have been thawed and re-frozen; Dry swabs; Stool in diapers; Unlabeled specimens

Minimum Test Volume