Children's Hospital Colorado Biochemical Genetics
Other Reference Test
1-5 mL Urine (Pre- and/or Post-Choline)
It is recommended to collect two urine specimens (Pre- and Post-Choline load) ordered separately, but one Post-Choline load is sufficient. For a Pre-Choline load specimen, 1-5 mL morning void urine, collected when the patient is eating an unrestricted diet and odor is present. For a Post-Choline load specimen, 1-5 mL morning void urine, should be collected about 12 hours after the patient has ingested choline bitartrate with a glass of orange juice or water (5 gm or 50000 mg). Freeze immediately after collection.
5 mL Urine (Pre- and/or Post-Choline)
1 mL Urine (Pre- and/or Post-Choline)
If possible, acidify the specimen to pH=4.0 before freezing. Complete a Children's Hospital Colorado Biochemical Genetics requisition and submit with specimen frozen Monday-Thursday only, no weekends of holidays. Clearly label specimens and requisitions Pre- and/or Post-Choline.