Methodology
Flow Cytometry
Specimen Type
Whole Blood or Bone Marrow
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
LAB01115
Lab Test Days
Daily
Standard TAT
4-5 Days
Collection Information
Specimen Required
10 mL Whole Blood or 4 mL Bone Marrow
Container Type
Lavender (EDTA)
Collection Instructions
Whole blood or bone marrow collected in sodium heparin (green-top) or ACD solution B (yellow-top) is also acceptable. Fresh (unfixed) tissue, Buffy coat, CSF, Biopsy, Amniotic fluid, Fluid, Cyst fluid, Gastric fluid, Pericardial fluid, Peritoneal fluid, Synovial fluid, or Vitreous fluid collected in a sterile screw cap container is also acceptable. Any tissue or body fluid is acceptable. Sample size is dependent upon leukocyte cellularity of sample. Minimum of 50,000 cells of interest in total volume of tissue or fluid. Absolutely no fixative can be added.
Processing Information - Lab Use Only
Transport Temperature
Refrigerate-OK/Ambient-OK
Specimen Stability
Room Temp: 72 Hours; Refrigerated: Tissue and Body Fluids Only; Frozen: Call Lab
Test Volume Required
10 mL Whole Blood or 4 mL Bone Marrow
Minimum Test Volume
3 mL Whole Blood or 1 mL Bone Marrow
Processing Instructions
Indicate source in comments and on specimen. If sending tissue, indicate Histology Specimen Number and source in comments and on specimen. Whole blood or Bone Marrow send immediately room temp. Tissue or body fluid send immediately refrigerated. DO NOT FREEZE.
Included Tests
CD3, CD7, CD13, CD19, CD20, CD33, CD34, CD45, CD64, CD45 is used for gating. Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker).
Comments
Indicate source in comments and on specimen. If sending tissue, indicate Histology Specimen Number and source in comments and on specimen.
CPT Codes
88184, 88185 (x8), 88188
Revised
02-09-15
Initials
SL