Methodology
Polymerase Chain Reaction (PCR); Fragment Analysis
Specimen Type
Whole Blood or Buccal Swabs
Performing Lab
UCSD CALM Immunogenetics (HLA)
Epic ID
LAB00135
Lab Test Days
Monday-Friday
Standard TAT
5-7 Days
Collection Information
Specimen Required
1-5 mL Whole Blood or 4 Buccal Swabs
Container Type
Yellow (ACD), Soultion A
Collection Instructions
Collect Monday-Friday before 1200. It is not recommended to collect on weekends or holidays. IF WBC is low, must call UCSD Immunogenetics 858-657-5776 for an adequate draw amount. Whole blood collected in an EDTA (lavender-top) tube is also acceptable. RCH BMT Transplant Department must submit a completed UCSD Immunogenetics requisition with specimen to lab. For Buccal Swab collection, ensure that the patient has not consumed any food or drink in the 30 minutes prior to sample collection.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-OK/Refrigerate-OK
Specimen Stability
Room Temp: 3 Days (48 Hours for Buccal Swabs) (preferred); Refrigerated: 7 Days (48 Hours for Buccal Swabs); Frozen: Unacceptable
Rejection Criteria
Lack of referral requisition; Grossly hemolyzed or severely clotted specimens; Specimens containing particulate matter or exhibiting obvious contamination; Commingled specimens; Specimens that have been improperly collected, stored, or transported; Specimens not received within 48 hours of collection; Specimens collected using tubes other than ACD Whole Blood Tubes (Yellow Top Vacutainer); EDTA and Sodium Heparin Tubes are accepted, but ACD Tubes are preferred; Serum specimens; Specimens that have been frozen; Specimens that are leaking or received in broken containers; Specimens received with insufficient volume for testing (< 1 mL); Unlabeled or mislabeled specimens
Test Volume Required
5 mL Whole blood or 4 Buccal Swabs
Minimum Test Volume
1 mL Whole blood or 4 Buccal Swabs
Processing Instructions
Submit a completed UCSD CALM Immunogenetics (HLA) requisition with specimen room temp Monday-Friday before 1400. Indicate whole blood or buccal swabs in accession comments and on requisition. Deliver to UCSD the same day as collected. If specimen is received without a completed UCSD Immunogenetics requisition, contact RCH BMT Coordinators at x223676.
Comments
Insurance authorization is required prior to draw for Outpatients. Ok to send on Inpatients.
CPT Codes
81265
Revised
12/19/25
Initials
SA