Photometric Clot Detection; Chromogenic Assay
Quest Diagnostics San Juan Capistrano
Other Reference Test
Wednesday and Saturday
(one-three) 1.8 mL or (one-two) 2.7 mL mL Blood
Light Blue (Sodium Citrate)
Room Temp: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 Year
Hemolysis; Received room temp;Received refrigerated; Received thawed
3 mL Platelet-Poor Plasma
1 mL Platelet-Poor Plasma
Platelet Poor Plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and send frozen.
If Factor VIII Activity, Clotting is >20, Factor VIII Activity, Chromogenic will be performed.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII. A patient must not be given any recombinant vWF or vWF concentrates prior to testing.