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Lab Test Dictionary

JAK2, Exon 12 Mutation Analysis (92474)


Polymerase Chain Reaction-based DNA Sequencing


Myeloproliferative neoplasm; PMF; Essential Thrombocythemia (ET); Polycythemia Vera (PV); ET; Primary Myelofibrosis (PMF); PV; JAK2 V617F

Specimen Type

Whole Blood or Bone Marrow

Performing Lab

Quest Diagnostics San Juan Capistrano

Epic ID

Other Reference Test

Lab Test Days


Standard TAT

5-7 Days

Collection Information

Specimen Required

3-4 mL Whole Blood or 1-3 mL Bone Marrow

Container Type

EDTA (Lavender-Top) Tube

Collection Instructions

Whole blood or bone marrow aspirate collected in a sodium heparin (green-top) tube; Fixed cell pellet collected in sterile leak-proof container; Extracted DNA from CLIA-certified laboratory collected in a sterile leak-proof container is also acceptable.

Processing Information - Lab Use Only

Transport Temperature

Room Temp-OK/Refrigerated-OK/Frozen-OK

Specimen Stability

Room Temp: 7 Days, Refrigerated: 7 Days (14 Days for Extracted DNA); Frozen: Unacceptable (1 Year for Extracted DNA)

Minimum Test Volume

3 mL blood or 1 mL bone marrow aspirate

Processing Instructions

Cell pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable. Do not reject specimens, send to laboratory for screening.


Insurance authorization required prior to collection for Outpatients. Ok to send on Inpatients.

CPT Codes