Methodology
Polymerase Chain Reaction-based DNA Sequencing
Synonyms
Myeloproliferative neoplasm; PMF; Essential Thrombocythemia (ET); Polycythemia Vera (PV); ET; Primary Myelofibrosis (PMF); PV; JAK2 V617F
Specimen Type
Whole Blood or Bone Marrow
Performing Lab
Quest Diagnostics San Juan Capistrano
Epic ID
Other Reference Test
Lab Test Days
Daily
Standard TAT
5-7 Days
Collection Information
Specimen Required
3-4 mL Whole Blood or 1-3 mL Bone Marrow
Container Type
EDTA (Lavender-Top) Tube
Collection Instructions
Whole blood or bone marrow aspirate collected in a sodium heparin (green-top) tube; Fixed cell pellet collected in sterile leak-proof container; Extracted DNA from CLIA-certified laboratory collected in a sterile leak-proof container is also acceptable.
Processing Information - Lab Use Only
Transport Temperature
Room Temp-OK/Refrigerated-OK/Frozen-OK
Specimen Stability
Room Temp: 7 Days, Refrigerated: 7 Days (14 Days for Extracted DNA); Frozen: Unacceptable (1 Year for Extracted DNA)
Minimum Test Volume
3 mL blood or 1 mL bone marrow aspirate
Processing Instructions
Cell pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable. Do not reject specimens, send to laboratory for screening.
Comments
Insurance authorization required prior to collection for Outpatients. Ok to send on Inpatients.
CPT Codes
81279
Revised
7/19/23
Initials
SA